Sex differences in glutathione metabolism and acetaminophen toxicity Tylenol's Impact on Glutathione and Liver Damage Myth busting, Acetaminophen version. "Avoid Acetaminophen (Tylenol) because it causes glutathione depletion". Truth?? As a pediatrician and ER doctor and as moms we're often asked about this. And we understand why: Is Tylenol Safe During Pregnancy? Understanding Risk Factors Seeking Health Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Clinically relevant therapeutic approaches against acetaminophen hepatotoxicity and acute liver failure ScienceDirect