Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. sagesteele @P_McCulloughMD While I dont know whether there is a connection between Tylenol and autism, I do believe that there is reason to STOP giving it to babies children. Glutathione is impacted by Sex differences in glutathione metabolism and acetaminophen toxicity Interaction with Ethanol Alcohol Metabolism Paracetamol (Acetaminophen) hepatotoxicity and its management. Just say NO to Tylenol